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polyclonal antibody against il-17f  (Pocono Rabbit Farm)

 
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    Pocono Rabbit Farm polyclonal antibody against il-17f
    <t>IL-17F</t> is produced <t>by</t> <t>IL-17–expressing</t> T cells. (A) 293T cells transfected with pcDNA–IL-17 <t>or</t> <t>pcDNA–IL-17F,</t> or vector only, were fixed and stained with the indicated antibodies. (B) CD4 + T cells from OT-II mice were differentiated into Th1, Th2, and Th17 lineages. On day 5 of culture, CD4 + T cells were restimulated with PMA and ionomycin and stained with appropriate antibodies. (C) Splenocytes were activated with PMA and Ionomycin for 5 h, and IL-17– and IL-17F–expressing cells were assessed by intracellular staining on CD4 + or TCRγδ + gates. (D) IL-17F and IL-17F–expressing cells in CNS infiltrates of mice with EAE, and in the lamina propria and intestinal intraepithelial lymphocytes were analyzed by intracellular staining with CD4 + gating. CNS infiltrates were isolated from perfused mice on day 12 after the second immunization. Data are representative of at least two independent experiments with similar results (percentages are shown).
    Polyclonal Antibody Against Il 17f, supplied by Pocono Rabbit Farm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+antibody+against+il-17f/polyclonal+antibody+against+il+17f/pmc02373839-198-0-7
    Average 90 stars, based on 1 article reviews
    polyclonal antibody against il-17f - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Regulation of inflammatory responses by IL-17F"

    Article Title: Regulation of inflammatory responses by IL-17F

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20071978

    IL-17F is produced by IL-17–expressing T cells. (A) 293T cells transfected with pcDNA–IL-17 or pcDNA–IL-17F, or vector only, were fixed and stained with the indicated antibodies. (B) CD4 + T cells from OT-II mice were differentiated into Th1, Th2, and Th17 lineages. On day 5 of culture, CD4 + T cells were restimulated with PMA and ionomycin and stained with appropriate antibodies. (C) Splenocytes were activated with PMA and Ionomycin for 5 h, and IL-17– and IL-17F–expressing cells were assessed by intracellular staining on CD4 + or TCRγδ + gates. (D) IL-17F and IL-17F–expressing cells in CNS infiltrates of mice with EAE, and in the lamina propria and intestinal intraepithelial lymphocytes were analyzed by intracellular staining with CD4 + gating. CNS infiltrates were isolated from perfused mice on day 12 after the second immunization. Data are representative of at least two independent experiments with similar results (percentages are shown).
    Figure Legend Snippet: IL-17F is produced by IL-17–expressing T cells. (A) 293T cells transfected with pcDNA–IL-17 or pcDNA–IL-17F, or vector only, were fixed and stained with the indicated antibodies. (B) CD4 + T cells from OT-II mice were differentiated into Th1, Th2, and Th17 lineages. On day 5 of culture, CD4 + T cells were restimulated with PMA and ionomycin and stained with appropriate antibodies. (C) Splenocytes were activated with PMA and Ionomycin for 5 h, and IL-17– and IL-17F–expressing cells were assessed by intracellular staining on CD4 + or TCRγδ + gates. (D) IL-17F and IL-17F–expressing cells in CNS infiltrates of mice with EAE, and in the lamina propria and intestinal intraepithelial lymphocytes were analyzed by intracellular staining with CD4 + gating. CNS infiltrates were isolated from perfused mice on day 12 after the second immunization. Data are representative of at least two independent experiments with similar results (percentages are shown).

    Techniques Used: Produced, Expressing, Transfection, Plasmid Preparation, Staining, Isolation

    IL-17F induces proinflammatory cytokines and chemokines in vitro via IL-17RA, TRAF6, and Act1. (A) MEFs were treated with 100 ng/ml IL-17 and IL-17F, and RNA samples were analyzed for expression of the indicated genes using real-time PCR. (B) MEFs from WT and IL-17RA KO mice were treated with 100 ng/ml IL-17 or IL-17F. After overnight incubation, cell supernatants were subjected for ELISA. After administration of recombinant IL-17 and IL-17F in WT and IL-17RA KO mice, neutrophil numbers were counted. The results shown are averaged from three animals in each group. (C) WT and TRAF6-deficient MEFs were treated with cytokines overnight. Culture supernatants were subjected for ELISA. (D) Real-time PCR was performed using cDNAs from WT and Act1-deficient MEFs treated with the indicated cytokines. p-values were calculated between cytokine treatment and medium alone (A) or between the groups indicated by a horizontal line (B–D) using an unpaired Student's t test. The experiments were repeated two to three times with consistent results. Results are mean ± SD. **, P < 0.005.
    Figure Legend Snippet: IL-17F induces proinflammatory cytokines and chemokines in vitro via IL-17RA, TRAF6, and Act1. (A) MEFs were treated with 100 ng/ml IL-17 and IL-17F, and RNA samples were analyzed for expression of the indicated genes using real-time PCR. (B) MEFs from WT and IL-17RA KO mice were treated with 100 ng/ml IL-17 or IL-17F. After overnight incubation, cell supernatants were subjected for ELISA. After administration of recombinant IL-17 and IL-17F in WT and IL-17RA KO mice, neutrophil numbers were counted. The results shown are averaged from three animals in each group. (C) WT and TRAF6-deficient MEFs were treated with cytokines overnight. Culture supernatants were subjected for ELISA. (D) Real-time PCR was performed using cDNAs from WT and Act1-deficient MEFs treated with the indicated cytokines. p-values were calculated between cytokine treatment and medium alone (A) or between the groups indicated by a horizontal line (B–D) using an unpaired Student's t test. The experiments were repeated two to three times with consistent results. Results are mean ± SD. **, P < 0.005.

    Techniques Used: In Vitro, Expressing, Real-time Polymerase Chain Reaction, Incubation, Enzyme-linked Immunosorbent Assay, Recombinant

    CC10–IL-17F mice develop lung inflammation and mucus hyperplasia. (A) MLE12 were treated with 100 ng/ml IL-17F for 4 h and subjected to real-time PCR analysis. (B) Schematic illustration of the construct in the IL-17F transgenic mice. hGH, human growth hormone. (C) RT-PCR detection of IL-17 and IL-17F mRNA in the lung. (D) Immunohistochemistry of lung sections from IL-17F transgenic mice. Frozen lung tissues were stained with CD4, CD8, B220, and CD11C. (E and F) H&E staining of lung sections from 5–7-mo-old IL-17F transgenic mice. High power magnification shows the accumulation of mixed inflammatory cells, macrophages, and Charcot-Leyden–like crystals. (G) PAS staining of transgenic mouse lung shows mucus overproduction. (H) RT-PCR of homogenized lung from IL-17F transgenic mice and age-matched WT. Data shown were repeated at least twice with similar results. Results are mean ± SD. Bars: (D and E) 100 μm; (F and G) 300 μm.
    Figure Legend Snippet: CC10–IL-17F mice develop lung inflammation and mucus hyperplasia. (A) MLE12 were treated with 100 ng/ml IL-17F for 4 h and subjected to real-time PCR analysis. (B) Schematic illustration of the construct in the IL-17F transgenic mice. hGH, human growth hormone. (C) RT-PCR detection of IL-17 and IL-17F mRNA in the lung. (D) Immunohistochemistry of lung sections from IL-17F transgenic mice. Frozen lung tissues were stained with CD4, CD8, B220, and CD11C. (E and F) H&E staining of lung sections from 5–7-mo-old IL-17F transgenic mice. High power magnification shows the accumulation of mixed inflammatory cells, macrophages, and Charcot-Leyden–like crystals. (G) PAS staining of transgenic mouse lung shows mucus overproduction. (H) RT-PCR of homogenized lung from IL-17F transgenic mice and age-matched WT. Data shown were repeated at least twice with similar results. Results are mean ± SD. Bars: (D and E) 100 μm; (F and G) 300 μm.

    Techniques Used: Real-time Polymerase Chain Reaction, Construct, Transgenic Assay, Reverse Transcription Polymerase Chain Reaction, Immunohistochemistry, Staining

    Analysis of T and B cell responses in IL-17 and IL-17F KO animals. IL-17F KO, IL-17 KO, and WT control mice were immunized with KLH in CFA (three per group). 7 d later, the mice were killed and spleens and blood were collect. (A) Splenocytes from the immunized mice were restimulated with KLH for 3 d, and cytokine expression was measured by ELISA. (B) KLH-specific antibodies were measured in the sera by ELISA. The sera were subject to a threefold serial dilution, and the antibody concentrations are shown as the mean for each group. Results are mean ± SD.
    Figure Legend Snippet: Analysis of T and B cell responses in IL-17 and IL-17F KO animals. IL-17F KO, IL-17 KO, and WT control mice were immunized with KLH in CFA (three per group). 7 d later, the mice were killed and spleens and blood were collect. (A) Splenocytes from the immunized mice were restimulated with KLH for 3 d, and cytokine expression was measured by ELISA. (B) KLH-specific antibodies were measured in the sera by ELISA. The sera were subject to a threefold serial dilution, and the antibody concentrations are shown as the mean for each group. Results are mean ± SD.

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay, Serial Dilution

    IL-17 but not IL-17F is required for the initiation of EAE. (A) EAE was induced in IL-17F KO, IL-17 KO, and WT control mice. Data shown are a combination of two independent experiments ( n = 10 for each group). (B) Infiltrates in the CNS from the EAE mice were isolated after perfusion on day 12 after the second immunization, and CD4 + and CD11b + cells were assessed by FACS. Horizontal bars indicate mean values. Data shown represent two independent experiments with similar results. (C) Chemokine expression in the CNS from the EAE mice was measured by real-time RT-PCR. (D) Splenocytes from the EAE mice were stimulated with MOG peptide, and cytokine expression levels were measured by ELISA. Data shown represent two independent experiments with similar results. (E) WT, IL-17 KO, and IL-17F KO mice were immunized with MOG/CFA and administered with pertussis toxin on the next day. 7 d after immunization, the mice were killed and IL-17–, IL-17F–, and IFN-γ–producing cells in the spleen and draining lymph nodes were analyzed by intracellular staining. Data shown are gated on CD4 + cells and are averaged from three to four mice in each group. Results are mean ± SD. *, P < 0.05; and **, P < 0.005 using the Student's t test. n.s., not significant.
    Figure Legend Snippet: IL-17 but not IL-17F is required for the initiation of EAE. (A) EAE was induced in IL-17F KO, IL-17 KO, and WT control mice. Data shown are a combination of two independent experiments ( n = 10 for each group). (B) Infiltrates in the CNS from the EAE mice were isolated after perfusion on day 12 after the second immunization, and CD4 + and CD11b + cells were assessed by FACS. Horizontal bars indicate mean values. Data shown represent two independent experiments with similar results. (C) Chemokine expression in the CNS from the EAE mice was measured by real-time RT-PCR. (D) Splenocytes from the EAE mice were stimulated with MOG peptide, and cytokine expression levels were measured by ELISA. Data shown represent two independent experiments with similar results. (E) WT, IL-17 KO, and IL-17F KO mice were immunized with MOG/CFA and administered with pertussis toxin on the next day. 7 d after immunization, the mice were killed and IL-17–, IL-17F–, and IFN-γ–producing cells in the spleen and draining lymph nodes were analyzed by intracellular staining. Data shown are gated on CD4 + cells and are averaged from three to four mice in each group. Results are mean ± SD. *, P < 0.05; and **, P < 0.005 using the Student's t test. n.s., not significant.

    Techniques Used: Isolation, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Staining

    IL-17 KO and IL-17F KO mice exhibited differential acute and chronic allergic responses. (A) Recruitment of neutrophils by FAP. WT, IL-17 KO, IL-17F KO, and IL-17RA KO mice were challenged once intranasally with either PBS or OVA with FAP. 18 h later, BALF cells were collected and stained with Gr.1 by FACS. Data shown are a combination of two independent experiments with similar results ( n = 6–8 mice per group). (B) Expression of chemokines. Whole-lung mRNA was collected and subjected to quantitative real-time RT-PCR. Data are expressed as the fold induction relative to PBS-challenged animals. (C–E). WT, IL-17, and IL-17F KO mice were subjected to an asthma model. (C) Cellular profiles in BAL fluid upon OVA challenge. Cells were harvested from BALF, stained by May-Grünwald-Giemsa, and counted under a microscope. Eos, eosinophil; Lymp, lymphocyte; Mac, macrophage; Neu, neutrophil. Data shown represent two independent experiments with consistent results ( n = 5). (D) Major basic protein 1 (MBP-1) and eosinophil peroxidase (EPO) expression in BAL cells. Real-time RT-PCR was performed using cDNA derived from BAL cells. CD11b expression in BAL cells was evaluated by FACS. Statistical analysis was performed between the indicated group and WT. (E) Expression of type 2 cytokines in lung lymph nodes and spleen. 3 d after culture in the presence of OVA, the levels of IL-4, IL-5, and IL-13 were measured by ELISA. Data shown in B, D, and E were repeated twice with similar results. Results are mean ± SD. *, P < 0.05; and **, P < 0.01 using the Student's t test. n.s., not significant.
    Figure Legend Snippet: IL-17 KO and IL-17F KO mice exhibited differential acute and chronic allergic responses. (A) Recruitment of neutrophils by FAP. WT, IL-17 KO, IL-17F KO, and IL-17RA KO mice were challenged once intranasally with either PBS or OVA with FAP. 18 h later, BALF cells were collected and stained with Gr.1 by FACS. Data shown are a combination of two independent experiments with similar results ( n = 6–8 mice per group). (B) Expression of chemokines. Whole-lung mRNA was collected and subjected to quantitative real-time RT-PCR. Data are expressed as the fold induction relative to PBS-challenged animals. (C–E). WT, IL-17, and IL-17F KO mice were subjected to an asthma model. (C) Cellular profiles in BAL fluid upon OVA challenge. Cells were harvested from BALF, stained by May-Grünwald-Giemsa, and counted under a microscope. Eos, eosinophil; Lymp, lymphocyte; Mac, macrophage; Neu, neutrophil. Data shown represent two independent experiments with consistent results ( n = 5). (D) Major basic protein 1 (MBP-1) and eosinophil peroxidase (EPO) expression in BAL cells. Real-time RT-PCR was performed using cDNA derived from BAL cells. CD11b expression in BAL cells was evaluated by FACS. Statistical analysis was performed between the indicated group and WT. (E) Expression of type 2 cytokines in lung lymph nodes and spleen. 3 d after culture in the presence of OVA, the levels of IL-4, IL-5, and IL-13 were measured by ELISA. Data shown in B, D, and E were repeated twice with similar results. Results are mean ± SD. *, P < 0.05; and **, P < 0.01 using the Student's t test. n.s., not significant.

    Techniques Used: Staining, Expressing, Quantitative RT-PCR, Microscopy, Derivative Assay, Enzyme-linked Immunosorbent Assay

    IL-17 and IL-17F differentially regulate DSS-induced colitis. (A) Acute colitis was induced in WT, IL-17 KO, and IL-17F KO mice by oral feeding of DSS ( n = 5–8 per group). Weight loss during colitis progression is shown. (B) Mice with colitis were killed on day 8, and the middle segment of the colon was fixed, sectioned, and stained with H&E. Bar, 1 mm. (C) Clinical scores are indicated based on microscopic examination of an epithelial lesion in the colon. (D) Chemokine mRNA expression was assessed in colon tissues by real-time RT-PCR. Data were expressed as the relative abundance of Actb. The experiments were repeated at least two to three times with consistent results. Results are mean values. *, P < 0.05; and **, P < 0.005 using the Student's t test.
    Figure Legend Snippet: IL-17 and IL-17F differentially regulate DSS-induced colitis. (A) Acute colitis was induced in WT, IL-17 KO, and IL-17F KO mice by oral feeding of DSS ( n = 5–8 per group). Weight loss during colitis progression is shown. (B) Mice with colitis were killed on day 8, and the middle segment of the colon was fixed, sectioned, and stained with H&E. Bar, 1 mm. (C) Clinical scores are indicated based on microscopic examination of an epithelial lesion in the colon. (D) Chemokine mRNA expression was assessed in colon tissues by real-time RT-PCR. Data were expressed as the relative abundance of Actb. The experiments were repeated at least two to three times with consistent results. Results are mean values. *, P < 0.05; and **, P < 0.005 using the Student's t test.

    Techniques Used: Staining, Expressing, Quantitative RT-PCR

    Related Articles

    Purification:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Produced:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Expressing:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Transfection:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Plasmid Preparation:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Staining:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Isolation:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    In Vitro:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Real-time Polymerase Chain Reaction:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Incubation:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Recombinant:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Construct:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Transgenic Assay:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Immunohistochemistry:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Serial Dilution:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Quantitative RT-PCR:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Microscopy:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Derivative Assay:

    Article Title: Regulation of inflammatory responses by IL-17F
    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.



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    polyclonal antibody against il-17f  (Pocono Rabbit Farm)
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    <t>IL-17F</t> is produced <t>by</t> <t>IL-17–expressing</t> T cells. (A) 293T cells transfected with pcDNA–IL-17 <t>or</t> <t>pcDNA–IL-17F,</t> or vector only, were fixed and stained with the indicated antibodies. (B) CD4 + T cells from OT-II mice were differentiated into Th1, Th2, and Th17 lineages. On day 5 of culture, CD4 + T cells were restimulated with PMA and ionomycin and stained with appropriate antibodies. (C) Splenocytes were activated with PMA and Ionomycin for 5 h, and IL-17– and IL-17F–expressing cells were assessed by intracellular staining on CD4 + or TCRγδ + gates. (D) IL-17F and IL-17F–expressing cells in CNS infiltrates of mice with EAE, and in the lamina propria and intestinal intraepithelial lymphocytes were analyzed by intracellular staining with CD4 + gating. CNS infiltrates were isolated from perfused mice on day 12 after the second immunization. Data are representative of at least two independent experiments with similar results (percentages are shown).
    Polyclonal Antibody Against Il 17f, supplied by Pocono Rabbit Farm, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+antibody+against+il-17f/polyclonal+antibody+against+il+17f/pmc02373839-198-0-7
    Average 90 stars, based on 1 article reviews
    polyclonal antibody against il-17f - by Bioz Stars, 2026-10
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    Image Search Results


    IL-17F is produced by IL-17–expressing T cells. (A) 293T cells transfected with pcDNA–IL-17 or pcDNA–IL-17F, or vector only, were fixed and stained with the indicated antibodies. (B) CD4 + T cells from OT-II mice were differentiated into Th1, Th2, and Th17 lineages. On day 5 of culture, CD4 + T cells were restimulated with PMA and ionomycin and stained with appropriate antibodies. (C) Splenocytes were activated with PMA and Ionomycin for 5 h, and IL-17– and IL-17F–expressing cells were assessed by intracellular staining on CD4 + or TCRγδ + gates. (D) IL-17F and IL-17F–expressing cells in CNS infiltrates of mice with EAE, and in the lamina propria and intestinal intraepithelial lymphocytes were analyzed by intracellular staining with CD4 + gating. CNS infiltrates were isolated from perfused mice on day 12 after the second immunization. Data are representative of at least two independent experiments with similar results (percentages are shown).

    Journal: The Journal of Experimental Medicine

    Article Title: Regulation of inflammatory responses by IL-17F

    doi: 10.1084/jem.20071978

    Figure Lengend Snippet: IL-17F is produced by IL-17–expressing T cells. (A) 293T cells transfected with pcDNA–IL-17 or pcDNA–IL-17F, or vector only, were fixed and stained with the indicated antibodies. (B) CD4 + T cells from OT-II mice were differentiated into Th1, Th2, and Th17 lineages. On day 5 of culture, CD4 + T cells were restimulated with PMA and ionomycin and stained with appropriate antibodies. (C) Splenocytes were activated with PMA and Ionomycin for 5 h, and IL-17– and IL-17F–expressing cells were assessed by intracellular staining on CD4 + or TCRγδ + gates. (D) IL-17F and IL-17F–expressing cells in CNS infiltrates of mice with EAE, and in the lamina propria and intestinal intraepithelial lymphocytes were analyzed by intracellular staining with CD4 + gating. CNS infiltrates were isolated from perfused mice on day 12 after the second immunization. Data are representative of at least two independent experiments with similar results (percentages are shown).

    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Techniques: Produced, Expressing, Transfection, Plasmid Preparation, Staining, Isolation

    IL-17F induces proinflammatory cytokines and chemokines in vitro via IL-17RA, TRAF6, and Act1. (A) MEFs were treated with 100 ng/ml IL-17 and IL-17F, and RNA samples were analyzed for expression of the indicated genes using real-time PCR. (B) MEFs from WT and IL-17RA KO mice were treated with 100 ng/ml IL-17 or IL-17F. After overnight incubation, cell supernatants were subjected for ELISA. After administration of recombinant IL-17 and IL-17F in WT and IL-17RA KO mice, neutrophil numbers were counted. The results shown are averaged from three animals in each group. (C) WT and TRAF6-deficient MEFs were treated with cytokines overnight. Culture supernatants were subjected for ELISA. (D) Real-time PCR was performed using cDNAs from WT and Act1-deficient MEFs treated with the indicated cytokines. p-values were calculated between cytokine treatment and medium alone (A) or between the groups indicated by a horizontal line (B–D) using an unpaired Student's t test. The experiments were repeated two to three times with consistent results. Results are mean ± SD. **, P < 0.005.

    Journal: The Journal of Experimental Medicine

    Article Title: Regulation of inflammatory responses by IL-17F

    doi: 10.1084/jem.20071978

    Figure Lengend Snippet: IL-17F induces proinflammatory cytokines and chemokines in vitro via IL-17RA, TRAF6, and Act1. (A) MEFs were treated with 100 ng/ml IL-17 and IL-17F, and RNA samples were analyzed for expression of the indicated genes using real-time PCR. (B) MEFs from WT and IL-17RA KO mice were treated with 100 ng/ml IL-17 or IL-17F. After overnight incubation, cell supernatants were subjected for ELISA. After administration of recombinant IL-17 and IL-17F in WT and IL-17RA KO mice, neutrophil numbers were counted. The results shown are averaged from three animals in each group. (C) WT and TRAF6-deficient MEFs were treated with cytokines overnight. Culture supernatants were subjected for ELISA. (D) Real-time PCR was performed using cDNAs from WT and Act1-deficient MEFs treated with the indicated cytokines. p-values were calculated between cytokine treatment and medium alone (A) or between the groups indicated by a horizontal line (B–D) using an unpaired Student's t test. The experiments were repeated two to three times with consistent results. Results are mean ± SD. **, P < 0.005.

    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Techniques: In Vitro, Expressing, Real-time Polymerase Chain Reaction, Incubation, Enzyme-linked Immunosorbent Assay, Recombinant

    CC10–IL-17F mice develop lung inflammation and mucus hyperplasia. (A) MLE12 were treated with 100 ng/ml IL-17F for 4 h and subjected to real-time PCR analysis. (B) Schematic illustration of the construct in the IL-17F transgenic mice. hGH, human growth hormone. (C) RT-PCR detection of IL-17 and IL-17F mRNA in the lung. (D) Immunohistochemistry of lung sections from IL-17F transgenic mice. Frozen lung tissues were stained with CD4, CD8, B220, and CD11C. (E and F) H&E staining of lung sections from 5–7-mo-old IL-17F transgenic mice. High power magnification shows the accumulation of mixed inflammatory cells, macrophages, and Charcot-Leyden–like crystals. (G) PAS staining of transgenic mouse lung shows mucus overproduction. (H) RT-PCR of homogenized lung from IL-17F transgenic mice and age-matched WT. Data shown were repeated at least twice with similar results. Results are mean ± SD. Bars: (D and E) 100 μm; (F and G) 300 μm.

    Journal: The Journal of Experimental Medicine

    Article Title: Regulation of inflammatory responses by IL-17F

    doi: 10.1084/jem.20071978

    Figure Lengend Snippet: CC10–IL-17F mice develop lung inflammation and mucus hyperplasia. (A) MLE12 were treated with 100 ng/ml IL-17F for 4 h and subjected to real-time PCR analysis. (B) Schematic illustration of the construct in the IL-17F transgenic mice. hGH, human growth hormone. (C) RT-PCR detection of IL-17 and IL-17F mRNA in the lung. (D) Immunohistochemistry of lung sections from IL-17F transgenic mice. Frozen lung tissues were stained with CD4, CD8, B220, and CD11C. (E and F) H&E staining of lung sections from 5–7-mo-old IL-17F transgenic mice. High power magnification shows the accumulation of mixed inflammatory cells, macrophages, and Charcot-Leyden–like crystals. (G) PAS staining of transgenic mouse lung shows mucus overproduction. (H) RT-PCR of homogenized lung from IL-17F transgenic mice and age-matched WT. Data shown were repeated at least twice with similar results. Results are mean ± SD. Bars: (D and E) 100 μm; (F and G) 300 μm.

    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Techniques: Real-time Polymerase Chain Reaction, Construct, Transgenic Assay, Reverse Transcription Polymerase Chain Reaction, Immunohistochemistry, Staining

    Analysis of T and B cell responses in IL-17 and IL-17F KO animals. IL-17F KO, IL-17 KO, and WT control mice were immunized with KLH in CFA (three per group). 7 d later, the mice were killed and spleens and blood were collect. (A) Splenocytes from the immunized mice were restimulated with KLH for 3 d, and cytokine expression was measured by ELISA. (B) KLH-specific antibodies were measured in the sera by ELISA. The sera were subject to a threefold serial dilution, and the antibody concentrations are shown as the mean for each group. Results are mean ± SD.

    Journal: The Journal of Experimental Medicine

    Article Title: Regulation of inflammatory responses by IL-17F

    doi: 10.1084/jem.20071978

    Figure Lengend Snippet: Analysis of T and B cell responses in IL-17 and IL-17F KO animals. IL-17F KO, IL-17 KO, and WT control mice were immunized with KLH in CFA (three per group). 7 d later, the mice were killed and spleens and blood were collect. (A) Splenocytes from the immunized mice were restimulated with KLH for 3 d, and cytokine expression was measured by ELISA. (B) KLH-specific antibodies were measured in the sera by ELISA. The sera were subject to a threefold serial dilution, and the antibody concentrations are shown as the mean for each group. Results are mean ± SD.

    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Serial Dilution

    IL-17 but not IL-17F is required for the initiation of EAE. (A) EAE was induced in IL-17F KO, IL-17 KO, and WT control mice. Data shown are a combination of two independent experiments ( n = 10 for each group). (B) Infiltrates in the CNS from the EAE mice were isolated after perfusion on day 12 after the second immunization, and CD4 + and CD11b + cells were assessed by FACS. Horizontal bars indicate mean values. Data shown represent two independent experiments with similar results. (C) Chemokine expression in the CNS from the EAE mice was measured by real-time RT-PCR. (D) Splenocytes from the EAE mice were stimulated with MOG peptide, and cytokine expression levels were measured by ELISA. Data shown represent two independent experiments with similar results. (E) WT, IL-17 KO, and IL-17F KO mice were immunized with MOG/CFA and administered with pertussis toxin on the next day. 7 d after immunization, the mice were killed and IL-17–, IL-17F–, and IFN-γ–producing cells in the spleen and draining lymph nodes were analyzed by intracellular staining. Data shown are gated on CD4 + cells and are averaged from three to four mice in each group. Results are mean ± SD. *, P < 0.05; and **, P < 0.005 using the Student's t test. n.s., not significant.

    Journal: The Journal of Experimental Medicine

    Article Title: Regulation of inflammatory responses by IL-17F

    doi: 10.1084/jem.20071978

    Figure Lengend Snippet: IL-17 but not IL-17F is required for the initiation of EAE. (A) EAE was induced in IL-17F KO, IL-17 KO, and WT control mice. Data shown are a combination of two independent experiments ( n = 10 for each group). (B) Infiltrates in the CNS from the EAE mice were isolated after perfusion on day 12 after the second immunization, and CD4 + and CD11b + cells were assessed by FACS. Horizontal bars indicate mean values. Data shown represent two independent experiments with similar results. (C) Chemokine expression in the CNS from the EAE mice was measured by real-time RT-PCR. (D) Splenocytes from the EAE mice were stimulated with MOG peptide, and cytokine expression levels were measured by ELISA. Data shown represent two independent experiments with similar results. (E) WT, IL-17 KO, and IL-17F KO mice were immunized with MOG/CFA and administered with pertussis toxin on the next day. 7 d after immunization, the mice were killed and IL-17–, IL-17F–, and IFN-γ–producing cells in the spleen and draining lymph nodes were analyzed by intracellular staining. Data shown are gated on CD4 + cells and are averaged from three to four mice in each group. Results are mean ± SD. *, P < 0.05; and **, P < 0.005 using the Student's t test. n.s., not significant.

    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Techniques: Isolation, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Staining

    IL-17 KO and IL-17F KO mice exhibited differential acute and chronic allergic responses. (A) Recruitment of neutrophils by FAP. WT, IL-17 KO, IL-17F KO, and IL-17RA KO mice were challenged once intranasally with either PBS or OVA with FAP. 18 h later, BALF cells were collected and stained with Gr.1 by FACS. Data shown are a combination of two independent experiments with similar results ( n = 6–8 mice per group). (B) Expression of chemokines. Whole-lung mRNA was collected and subjected to quantitative real-time RT-PCR. Data are expressed as the fold induction relative to PBS-challenged animals. (C–E). WT, IL-17, and IL-17F KO mice were subjected to an asthma model. (C) Cellular profiles in BAL fluid upon OVA challenge. Cells were harvested from BALF, stained by May-Grünwald-Giemsa, and counted under a microscope. Eos, eosinophil; Lymp, lymphocyte; Mac, macrophage; Neu, neutrophil. Data shown represent two independent experiments with consistent results ( n = 5). (D) Major basic protein 1 (MBP-1) and eosinophil peroxidase (EPO) expression in BAL cells. Real-time RT-PCR was performed using cDNA derived from BAL cells. CD11b expression in BAL cells was evaluated by FACS. Statistical analysis was performed between the indicated group and WT. (E) Expression of type 2 cytokines in lung lymph nodes and spleen. 3 d after culture in the presence of OVA, the levels of IL-4, IL-5, and IL-13 were measured by ELISA. Data shown in B, D, and E were repeated twice with similar results. Results are mean ± SD. *, P < 0.05; and **, P < 0.01 using the Student's t test. n.s., not significant.

    Journal: The Journal of Experimental Medicine

    Article Title: Regulation of inflammatory responses by IL-17F

    doi: 10.1084/jem.20071978

    Figure Lengend Snippet: IL-17 KO and IL-17F KO mice exhibited differential acute and chronic allergic responses. (A) Recruitment of neutrophils by FAP. WT, IL-17 KO, IL-17F KO, and IL-17RA KO mice were challenged once intranasally with either PBS or OVA with FAP. 18 h later, BALF cells were collected and stained with Gr.1 by FACS. Data shown are a combination of two independent experiments with similar results ( n = 6–8 mice per group). (B) Expression of chemokines. Whole-lung mRNA was collected and subjected to quantitative real-time RT-PCR. Data are expressed as the fold induction relative to PBS-challenged animals. (C–E). WT, IL-17, and IL-17F KO mice were subjected to an asthma model. (C) Cellular profiles in BAL fluid upon OVA challenge. Cells were harvested from BALF, stained by May-Grünwald-Giemsa, and counted under a microscope. Eos, eosinophil; Lymp, lymphocyte; Mac, macrophage; Neu, neutrophil. Data shown represent two independent experiments with consistent results ( n = 5). (D) Major basic protein 1 (MBP-1) and eosinophil peroxidase (EPO) expression in BAL cells. Real-time RT-PCR was performed using cDNA derived from BAL cells. CD11b expression in BAL cells was evaluated by FACS. Statistical analysis was performed between the indicated group and WT. (E) Expression of type 2 cytokines in lung lymph nodes and spleen. 3 d after culture in the presence of OVA, the levels of IL-4, IL-5, and IL-13 were measured by ELISA. Data shown in B, D, and E were repeated twice with similar results. Results are mean ± SD. *, P < 0.05; and **, P < 0.01 using the Student's t test. n.s., not significant.

    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Techniques: Staining, Expressing, Quantitative RT-PCR, Microscopy, Derivative Assay, Enzyme-linked Immunosorbent Assay

    IL-17 and IL-17F differentially regulate DSS-induced colitis. (A) Acute colitis was induced in WT, IL-17 KO, and IL-17F KO mice by oral feeding of DSS ( n = 5–8 per group). Weight loss during colitis progression is shown. (B) Mice with colitis were killed on day 8, and the middle segment of the colon was fixed, sectioned, and stained with H&E. Bar, 1 mm. (C) Clinical scores are indicated based on microscopic examination of an epithelial lesion in the colon. (D) Chemokine mRNA expression was assessed in colon tissues by real-time RT-PCR. Data were expressed as the relative abundance of Actb. The experiments were repeated at least two to three times with consistent results. Results are mean values. *, P < 0.05; and **, P < 0.005 using the Student's t test.

    Journal: The Journal of Experimental Medicine

    Article Title: Regulation of inflammatory responses by IL-17F

    doi: 10.1084/jem.20071978

    Figure Lengend Snippet: IL-17 and IL-17F differentially regulate DSS-induced colitis. (A) Acute colitis was induced in WT, IL-17 KO, and IL-17F KO mice by oral feeding of DSS ( n = 5–8 per group). Weight loss during colitis progression is shown. (B) Mice with colitis were killed on day 8, and the middle segment of the colon was fixed, sectioned, and stained with H&E. Bar, 1 mm. (C) Clinical scores are indicated based on microscopic examination of an epithelial lesion in the colon. (D) Chemokine mRNA expression was assessed in colon tissues by real-time RT-PCR. Data were expressed as the relative abundance of Actb. The experiments were repeated at least two to three times with consistent results. Results are mean values. *, P < 0.05; and **, P < 0.005 using the Student's t test.

    Article Snippet: Polyclonal antibody against IL-17F was made by PRF&L by immunizing rabbits with purified IL-17F–Ig fusion proteins.

    Techniques: Staining, Expressing, Quantitative RT-PCR